GFAP Mouse, Unlabeled, Clone: 4A11, 1B4, 2E1, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 556330

Catalog No. BDB556330

Description

GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. BD Pharmingen offers a panel of monoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Bovine spinal cord homogenate was used as immunogen for these clones. Clones 4A11, 1B4, and 2E1 have broad species reactivity, recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken. The cocktail preparation was made by combining all three antibodies in equal concentrations. The antibodies are routinely tested by immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue samples, which were subjected to pretreatment with citrate or tissue unmasking fluid (see figure). Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Mouse
Clone: 2E1, 1B4, 4A11
Species Reactivity: Rat

Immunofluorescence, Immunohistochemistry, Western Blotting

Specifications

• Antigen: GFAP
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Immunohistochemistry (Paraffin)
• Clone: 4A11, 1B4, 2.00E+01
• Conjugate: Unconjugated
• Host Species: Mouse
• Quantity: 0.5 mg
• Research Discipline: Cell Biology
• Target Species: Bovine, Canine, Chicken, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
• Isotype: IgG2b

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.