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Description
GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. BD Biosciences Pharmingen offers a panel of monoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Clone 4A11 is specific for GFAP. Bovine spinal cord homogenate was used as immunogen. This antibody has broad species reactivity, recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken. 4A11 is particularly useful for identifying GFAP in immunohistochemistry of frozen and formalin-fixed, paraffin-embedded brain tissue sections. Additional applications include western blot analysis and indirect immunofluorescence of tissue-cultured cells. BD Biosciences Pharmingen offers additional GFAP specific antibodies: clone 2E1 (Mfr. No. 556329), 1B4 (Mfr. No. 556328), and clones 1B4, 4A11, 2E1 combined and available as a “cocktail” (Mfr. No. 556330).
Host Species: Mouse
Clone: 4A11
Isotype: IgG2b, κ
Species Reactivity: Human
Immunogen: Cow spinal cord homogenate
Immunofluorescence, Immunohistochemistry, Western Blotting
Specifications
Specifications
| Antigen | GFAP |
| Applications | Immunohistochemistry |
| Classification | Monoclonal |
| Clone | 4A11 |
| Concentration | 0.5mg/mL |
| Conjugate | Unconjugated |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Cow spinal cord homogenate |
| Purification Method | Affinity Purified |
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Safety and Handling
For Research Use Only.
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