GITR Rat anti-Mouse, NA/LE, Unlabeled, Clone: DTA-1, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 558213

Catalog No. BDB558213

Description

The DTA-1 antibody reacts with GITR [Glucocorticoid-induced Tumor necrosis factor (TNF) receptor family-Related], a 66-70kDa homodimer glycoprotein that is a member of the TNF receptor superfamily and is also known as TNFRSF18. As its name implies, GITR expression was first detected in T lymphocytes that had been treated with dexamethasone, a glucocorticoid. In normal naive mice, GITR is expressed at moderate levels on CD25-positive/CD4-positive/CD8a-negative thymocytes and on CD25-positive/CD4-positive/CD45RB-low splenocytes. It is also expressed at low levels on splenic CD25-negative/CD4-positive/CD45RB-low T lymphocytes, B lymphocytes, macrophages, and dendritic cells. Activation of T and B lymphocytes upregulates GITR expression. GITR is a costimulatory receptor that plays an important role in Regulatory T (Treg)-cell functions, and a GITR Ligand has been detected on B lymphocytes, macrophages, and dendritic cells. mAb DTA-1 abrogates suppression by Treg cells without affecting their proliferative response, while it is co-stimulatory for T lymphocytes that are not Treg cells.

Host Species: Rat
Clone: DTA-1
Isotype: IgG2b, λ
Cross Reactivity: Bovine, Mouse

Blocking Assay, Flow Cytometry

Specifications

• Antigen: GITR
• Classification: Monoclonal
• Concentration: 1.0mg/mL
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Immunogen: Mouse CD25+ CD4+ T Cell Line
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2b

• Applications: Flow Cytometry
• Clone: DTA-1
• Conjugate: Unconjugated
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.