GRP78, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA137806
The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. GRP78 is localized in the endoplasmic reticulum where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains.This antibody is predicted to react with bovine, chicken, canine, amphibian, mouse and rat based on sequence homology.Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast carcinoma.
|TBS with 1% BSA and <0.1% sodium azide; pH 7.6|
|BIP, Heat Shock 70kDa protein 5, GRP-78, 78kDa glucose-regulated protein, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, immunoglobulin heavy chain-binding protein|
|4° C, do not freeze, do not freeze|
|Synthetic peptide corresponding to near N-terminus of human GRP78|
|Antigen affinity chromatography|
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