MilliporeSigma™ anti-Glutamate Decarboxylase, 65kDa isoform, Clone: GAD-6, ™ (Chemicon™)

Supplier:  MilliporeSigma™ MAB351/DEL

Catalog No. 50-175-315

Description

Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65kDa and 67kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585aa), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594aa.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, P. et al. 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, L. et al. 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk et al., 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.

Immunohistochemistry, Western Blotting

Specifications

• Antigen: Glutamate Decarboxylase, 65kDa isoform
• Conjugate: Unconjugated
• Immunogen: Purified rat brain glutamic acid decarboxylase.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Form: Semi-purified

• Classification: Monoclonal
• Host Species: Mouse
• Quantity: 100 μg
• Research Discipline: Neuroscience
• Target Species: Human, Rat
• Isotype: IgG2a