GRB2 Mouse, Unlabeled, Clone: 81, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 610111

Catalog No. BDB610111

Description

Growth-factor Receptor-Bound Protein 2 (GRB2) was isolated by using the EGF receptor C-terminus as a probe in the screening of λgt11 expression libraries. This 24kDa GRB2 protein is ubiquitously expressed and consists of one SH2 domain flanked by two SH3 domains. Its ability to bind proteins through these domains has prompted much investigation of its role as an adaptor protein. Sos, a Ras GDP/GTP exchange protein, is constitutively bound to the SH3 domain of GRB2. Following growth factor stimulation, receptor tyrosine kinases autophosphorylate, creating a binding site for the GRB2 SH2 domain. Alternatively, GRB2 interacts with the receptor-bound active Shc protein.Through these interactions, the GRB2/Sos complex is translocated to the membrane where Sos activates membrane-bound Ras to initiate the Ras signaling pathway. Other proteins that similarly interact with GRB2 include Cbl, PTP1D, and Dynamin. However,the mechanisms through which these associations impact cellular responses remain to be discovered.

Host Species: Mouse
Clone: 81
Isotype: IgG1
Species Reactivity: Rat
Immunogen: Rat GRB2 aa. 1-217
Formula Weight [Chemical]: 24kDa

Bioimaging, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: GRB2
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Rat GRB2 aa. 1-217
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Canine, Chicken, Frog, Human, Mouse, Rat
• Isotype: IgG1

• Applications: Western Blot
• Clone: 81
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.