MilliporeSigma™ anti-GSTT1, Polyclonal

Catalog No.	Quantity	Conjugate
ABS1653MI	100 μL	Unconjugated

Catalog No. ABS1653MI Supplier MilliporeSigma™ Supplier No. ABS1653

Description

Rabbit Polyclonal Antibody

Specifically detects GSTT1 Clone: in Human, Mouse, Rat samples, and it is validated for Immunohistochemistry (Paraffin), Western Blotting

Glutathione S-transferase theta-1 (EC 2.5.1.18; UniProt P30711; also known as Glutathione S-transferase T1-1, GST class-theta-1, GST T1-1) is encoded by the GSTT1 gene (Gene ID 2952) in human. Glutathione S-transferases constitute a family of metabolic isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist as either homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (24.5kDa), Yk (25kDa), Ya1 (25.5kDa), Ya2 (25.5kDa), Yn (26.5kDa), Yb1 (27kDa), Yb2 (27kDa) and Yc (28.5kDa). The Y designation refers to the Y fraction (as apposed to the X and Z fractions) they were found in during purification by Sephadex G75 chromatography. Two GST superfamilies exist that comprise either cytosolic or membrane-bound proteins. Evidence suggests that cytosolic GST increases bodily resistance to carcinogens, products of oxidative stress, environmental pollutants and certain anti-cancer drugs. The cytosolic GSTs are further subdivided into eight classes. namely alpha (GSTA), mu (GSTM), pi (GSTP), sigma, theta (GSTT), kappa (GSTK), zeta (GSTZ), and omega (GSTO). GSTT1 exists as a homodimer, the subunit is referred to as subunit 5 in rat species, and T1-1 theta subunit in human.

Specifications

• Antigen: GSTT1
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Rabbit polyclonal antibody in serum with 0.05% sodium azide.
• Gene Accession No.: P30711
• Gene Symbols: GSTT1
• Host Species: Rabbit
• Immunogen: His-tagged recombinant full-length human GSTT1.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_000844
• Test Specificity: Expected to react with both spliced isoforms reported by UniProt (P30711). Note that not all human samples are positive for GSTT1 expression. It has been estimated that approx. 18% of the Caucasian, and as much as 60% of the Chinese and Korean populations are GSTT1-null (Katoh, T., et al. (1996) Carcinogenesis. 17(9):1855-1859; Lear, J.T., et al. (1996) Carcinogenesis. 17(9):1891-1896;).
• Target Species: Human, Mouse, Rat
• Content And Storage: −20°C for one year
• Form: Serum

For Research Use Only