H-2D[d] Mouse anti-Mouse, Unlabeled, Clone: 34-2-12, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 553577

Catalog No. BDB553577

Description

The 34-2-12 antibody (also known as 34-2-12S) recognizes the α3 domain of the H-2D[d]. The binding of the antibody to its epitope isindependent of the α1 and α2 domains and β2 microglobulin. It cross-reacts with cells of the C3H.LG/Ckc strain. Reactivity with otherhaplotypes (eg, b, f, k, p, q, r, s) has not been observed. Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 toimmobilized H-2D[d]. However, further studies utilizing this mAb indicate that the α3 domain is not involved in the interaction betweenLy-49A, or Ly-49G2, and H-2D[d]. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Mouse
Clone: 34-2-12
Isotype: IgG2a κ
Species Reactivity: Mouse
Immunogen: (C57BL/6 x DBA/2)F1 mouse splenocytes

Flow Cytometry, Immunoprecipitation

Specifications

• Antigen: H-2D[d]
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: (C57BL/6 x DBA/2)F1 mouse splenocytes
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: 34-2-12
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.