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H2AX (pS139) Mouse, Alexa Fluor 488, Clone: N1-431, BD

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Description

Mouse Monoclonal Antibody

Histones are highly basic proteins that complex with DNA to form chromatin. The H2AX histone (∽15kDa calculated molecular weight) is a member of the H2A histone family whose members are components of nucleosomal histone octamers. Double-stranded breaks in DNA caused by replication errors, apoptosis, or other physiological processes (including, immunoglobulin and TCR gene recombinations) and DNA damage caused by ionizing radiation, UV light, or cytotoxic agents lead to phosphorylation of H2AX on serine 139. H2AX (pS139) is also referred to as H2AX (pS140) when the N-terminal methionine that is normally excised during posttranslational processing is included in amino acid sequence numbering. Kinases such as ataxia telangiectasia mutated (ATM) or ATM-Rad3-related (ATR) phosphorylate H2AX to induce its function. Phosphorylated H2AX (also termed, γ-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites. In this way, phosphorylated H2AX promotes DNA repair and maintains genomic stability and thus helps prevent oncogenic transformations. Immunofluorescent staining and bioimaging analysis of cultured cells can be used to readily identify H2AX (pS139)-containing foci. As such, H2AX (pS139) immunofluorescence localization serves as a biomarker for nuclear sites of DNA damage (e.g., double-stranded DNA breaks) in affected cells.

Host Species: Mouse
Clone: N1-431
Isotype: IgG1 κ
Species Reactivity: Human
Immunogen: Phosphorylated Human H2AX Peptide

Bioimaging, Intracellular Staining

TRUSTED_SUSTAINABILITY

Specifications

Antigen H2AX (pS139)
Applications In vitro Assay
Classification Monoclonal
Clone N1-431
Conjugate Alexa Fluor 488
Description H2A.X; H2A/X; H2AFX; HIST5-2AX; gamma-H2AX; γ-H2AX; H2AX (pS140)
Formulation Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Phosphorylated Human H2AX Peptide
Purification Method Affinity Purified
Quantity 100 Tests
Regulatory Status RUO
Research Discipline Cell Biology
Primary or Secondary Primary
Target Species Human, Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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