H3 (pS28) Rat, V450, Clone: HTA28, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 560606

Catalog No. BDB560606

Description

Histones are highly basic proteins that complex with DNA to form chromatin. Histone H3 is a ∽15kDa protein that is phosphorylated at serine 28 (S28), S10, and/or threonine 11 during mammalian cell mitosis and meiosis. The phosphorylation sites are located in the N-terminal tail, a region that is outside of the chromatin fiber and is thus accessible for interactions with agents that may regulate chromatin or specific gene activities. The phosphorylation states of the two serine sites during the cell cycle are highly regulated by Aurora B kinase and a PP1 phosphatase: S10 is in the phosphorylated state from late G2 phase to anaphase, while S28 is phosphorylated from prophase to anaphase. Furthermore, phosphorylation of histone H3 S28 may be mediated by other kinases in response to external stimuli. Evidence suggests that histone phosphorylation is involved in the regulation of chromosome condensation, cell division, and gene transcription. The HTA28 monoclonal antibody reacts with histone H3 phosphorylated at S28 in its N-terminal tail. It does not recognize the unphosphorylated protein. HTA28 has been predicted to be reactive on human, rat, hamster, and cow samples. Investigators are advised that the BD Hoirzon™ V450 Rat Anti-Histone H3 (pS28) antibody (clone HTA28) is not routinely tested on human, rat, hamster, and cow samples. The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates

Intracellular Staining

Specifications

• Antigen: H3 (pS28)
• Classification: Monoclonal
• Conjugate: Violet Fluor 450 (V450)
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: HTA28
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: Phosphorylated Human Histone H3 Peptide
• Quantity: 50 Tests
• Research Discipline: Cell Biology
• Target Species: Bovine, Hamster, Human, Mouse, Rat
• Isotype: IgG2a κ