MilliporeSigma™ HAF/SART1, Rabbit, Unlabeled, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABC1431

Catalog No. ABC1431MI

Description

U4/U6.U5 tri-snRNP-associated protein 1 (UniProt O43290; also known as Allergen Hom s 1, HAF, hSART-1, hSnu66, Hypoxia-associated factor, SART-1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110kDa protein) is encoded by the SART1 (also known as HAF) gene (Gene ID 9092) in human. The hypoxia-associated factor (HAF) is an E3 ubiquitin ligase that mediates the ubiquitination and degradation of hypoxia-inducible factor 1alpha (HIF-1alpha). HAF also binds to HIF-2alpha and promotes HIF-2 transactivation instead of promoting HIF-2alpha degradation. In addition, hypoxia-dependent HAF SUMOylation enhances HAF interaction with HIF-2alpha and induction of HIF-2 target genes transcription, such as OCT-3/4, SOX2 and NANOG. HAF therefore promotes stem cell characteristics, aggressive tumor growth and invasion by switching cells from HIF-1alpha- to HIF-2alpha-dependent signaling. HAF overexpression enhances glioblastoma cells that express both HIF-1 and HIF-2alpha to initiate tumors as intracranial xenografts in mice. On the other hand, HAF overexpression results in decreased xenograft tumor growth of HT29 colon carcinoma cells that only express HIF-1alpha.

Specifications

• Antigen: HAF/SART1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SART1
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Target specificity was confirmed by Western blotting analysis of HAF overexpression and HAF shRNA-mediated HAF knockdown in various human cancer cells (Koh, M.Y., et al. (2008). Mol. Cell. Biol. 28(23):7081-7095).
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• Applications: Immunoprecipitation, Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
• Gene Accession No.: O43290
• Immunogen: GST-tagged recombinant human HAF/SART1 C-terminal fragment.
• Quantity: 100 μL
• Research Discipline: Apoptosis & Cancer
• Gene ID (Entrez): NP_005137
• Target Species: Human
• Form: Serum

For Research Use Only. Not for use in diagnostic procedures.