Invitrogen™ HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 550

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA516010

Catalog No. PIMA516010

Description

In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended. Suggested positive control: Cos-7 nuclear extracts.

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

Specifications

• Antigen: HIF1A
• Classification: Monoclonal
• Concentration: 0.68 mg/mL
• Formulation: 50mM sodium borate with 0.05% sodium azide
• Gene Accession No.: O35800, Q16665, Q61221, Q9I8A9, Q9XTA5
• Gene Symbols: hif-1a, HIF1A, hif1a.L
• Immunogen: Fusion protein containing amino acids 432-528 of human HIF-1alpha.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Rabbit, Monkey, Bovine, Sheep, Pig, Feline, Frog, Ferret
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: H1alpha67
• Conjugate: DyLight 550
• Gene: HIF1A
• Gene Alias: AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100009579, 100337573, 101083686, 15251, 281814, 29560, 3091, 380141, 396696, 443519, 445838, 480348
• Content And Storage: 4°C, store in dark
• Form: Liquid