HIF-1β/ARNT1 Mouse, Unlabeled, Clone: 29, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 611078

Catalog No. BDB611078

Description

The Ah-receptor (AHR) is a ligand activated transcription factor that mediates the biological effects of agonists. AHR dimerizes with a structurally related protein known as ARNT (arylhydrocarbon-receptor nuclear transducer). This heterodimer binds enhancer elements and induces the expression of target genes, specifically those involved in the metabolism of xenobiotics. ARNT1 and ARNT2 are members of the basic-helix-loop-helix-PAS family of heterodimeric transcription factors, which also includes AHR, hypoxia-inducible factor-1α (HIF-1α), and the Drosophila single-minded protein (Sim). While ARNT2 expression is limited to brain and kidney, ARNT1 exhibits ubiquitous
expression. A targeted disruption of the Arnt locus in the mouse yields embryonic stem cells that fail to activate genes that normally respond to low oxygen tension. Arnt -/- embryos do not survive and show defective angiogenesis of the yolk sac and branchial arches, stunted development, and wasting. Thus, in addition to its regulation of xenobiotic metabolism genes, ARNT is thought to induce developmental gene expression resulting in vascularization of the developing embryo.

Immunofluorescence, Western Blotting

Specifications

• Antigen: HIF-1β/ARNT1
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Human HIF-1β/ARNT1 aa. 461-574
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Human, Mouse, Rat
• Isotype: IgG1

• Applications: Western Blot
• Clone: 29
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.