MilliporeSigma anti-hMre-11, Polyclonal
Rabbit Polyclonal Antibody
Manufacturer: MilliporeSigma PC388100UG
Specifically detects hMre-11 Clone: in Human, Mouse, Rat samples, and it is validated for Immunofluorescence, ImmunoprecipitationDouble strand breaks (DSB) in DNA are repaired by an evolutionarily conserved complex that includes the proteins Mre11 and Rad50. Both of these proteins are essential since nullizygous embryos die in utero, consistent with the fact that they both participate in two distinct processes: homologous recombination and non-homologous end joining. The Mre11 gene encodes a 680 amino acid protein that migrates on SDS/with an apparent mobility of~85kDa. Among many different functions ascribed to it, Mre11 exhibits a Mn2+-dependent 3'-5' exonuclease activity on double stranded DNA and a single-stranded endonuclease that facilitates repair of double strand breaks in DNA. Activation of Mre11 may involve post-translational modification since hyperphosphorylation is observed upon DNA damage. Efficient repair of DSBs in mammalian cells appears to require formation of nuclear foci comprised of Mre11, Rad50, and p95NBS1. These foci are observed following induction of DSBs with ionizing radiation, and ionizing radiation-induced foci (IRIF) containing Mre11 appear in a radiation dose dependent manner that slowly dissipates over time, presumably after DSB repair is completed. IRIF containing Mre11 do not form in cells lacking p95NBS1; and in cells lacking ATM, the number of IRIF is dramatically reduced. While Mre11's catalytic activites are essential for repair of DSBs, the Mre11/Rad50/p95NBS1 appears to function as a primary detector of DNA damage.
|human hMre11 protein|
|2°C to 8°C|
|Human, Murine, Rat|
|In 50mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.|
|Recognizes the ∽85kDa hMre-11 protein in IMR90 and K562 cells.|
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