MilliporeSigma™ anti-HNE-Michael Adducts, Reduced, Polyclonal

Catalog No.	Quantity	Conjugate
39-320-7100UL	100 μL	Unconjugated

Catalog No. 39-320-7100UL Supplier MilliporeSigma™ Supplier No. 393207100UL

Description

Rabbit Polyclonal Antibody

Specifically detects HNE-Michael Adducts, Reduced Clone: in Many Species samples, and it is validated for ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immuno Blotting

The CNS is enriched in polyunsaturated fatty acids and is therefore particularly vulnerable to lipid peroxidation. 4-Hydroxy-2-noneal (HNE) is one of the major lipid peroxidation products with cytotoxic and mutagenic activity. It reacts with protein residues such as histidine to generate stable Michael adducts. HNE has been shown to inhibit neurite outgrowth and disrupt microtubules and tubulin modification, suggesting it may play a role in Alzheimer's disease.

Specifications

• Antigen: HNE-Michael Adducts, Reduced
• Applications: ELISA, Immunoblot, Immunoblot, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Serum diluted in 50% glycerol, pH 7.5.
• Host Species: Rabbit
• Immunogen: HNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such astrans-2-noneal.
• Target Species: Multi-species
• Content And Storage: ≤ -70°C
• Form: Diluted
• Isotype: IgG

For Research Use Only