Invitrogen™ PCBP2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA522350

Catalog No. PIPA522350

Description

Recommended positive controls: 293T, A431, Jurkat, Raji, mouse liver. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (98%), Pig (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: PCBP2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PCBP2
• Gene Alias: Alpha CP2; Alpha-CP2; alphaCP-2; AW412548; CBP; CTBP; fb36h02; Heterogeneous nuclear ribonucleoprotein E2; heterogeneous nuclear ribonucleoprotein X; heterogenous nuclear ribonucleoprotein E2; hnRNP E2; hnRNP X; HNRNPE2; hnRNP-E2; Hnrnpx; HNRPE2; Hnrpx; MGC110998; Pcbp2; pcbp2 protein; PCBP3; poly(rC) binding protein 2; poly(rC)-binding protein 2; Putative heterogeneous nuclear ribonucleoprotein X; rCbinding protein 2; wu:fb36h02; zgc:55964
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18521, 363005, 5094
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q15366, Q61990
• Gene Symbols: PCBP2
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 193 of PCBP2 (Uniprot ID#Q15366).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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