hRAD9 Mouse, Unlabeled, Clone: 56, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 611324

Catalog No. BDB611324

Description

Cell cycle checkpoints are regulatory mechanisms that prevent cell cycle progression in the presence of DNA damage or incompletely replicated DNA. Many of the genes required for cell-cycle arrest are also involved in DNA repair, apoptosis, and induction of transcription. In yeast and humans, hRAD9 plays a role in cell cycle arrest during the G2 phase before entry into mitosis. Phosphorylated hRAD9 is found in the nucleus after DNA damage, and forms DNA damage-responsive complexes with other putative checkpoint control proteins, such as
hRAD1 and hHUS1. Expression of hRAD9 in S. pombe rad9::ura4⁺ cells restores resistance to the DNA synthesis inhibitor hydroxyurea and gamma rays. In addition, hRAD9 binds the anti-apoptotic proteins, Bcl-2 and Bcl-xL , and antisense hRAD9 RNA suppresses DNA-damage induced cell death. Thus, hRAD9 may be an important component of protein complexes that regulate cell cycle progression, as well as apoptosis, in response to DNA damage.

Host Species: Mouse
Clone: 56
Isotype: IgG1
Species Reactivity: Human
Immunogen: Human hRAD9 aa. 264-370
Formula Weight [Chemical]: 60kDa

Immunofluorescence, Western Blotting

Specifications

• Antigen: hRAD9
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Human hRAD9 aa. 264-370
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Human, Mouse, Rat
• Isotype: IgG1

• Applications: Western Blot
• Clone: 56
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov