MilliporeSigma™ anti- HSPA8/Hsc70 Clone: N69,

Catalog No.	Quantity	Conjugate
MABE1120MI	100 μg	Unconjugated

Catalog No. MABE1120MI Supplier MilliporeSigma™ Supplier No. MABE1120

Description

Mouse Monoclonal Antibody

Specifically detects HSPA8/Hsc70 Clone: N69 in Bovine, Human, Mouse, Rat samples, and it is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blotting

Heat shock cognate 71kDa protein (UniProt P19120; also known as Heat shock 70kDa protein 8, HSC70, HSC71, HSC73) is encoded by the HSPA8 (also known as HSC70, HSC73) gene (Gene ID 281831) in bovine species. The mammalian HSP70 family consists of the mitochondrial HSP75, the endoplasmic HSP78, the cytoplasmic HSC70 (a.k.a. HSC73 and HSP73) and its inducible counterpart HSP72 (a.k.a. HSP70). The latter two have similar structures and properties, and both of them bind misfolded, newly synthesized polypeptides in an ATP-dependent manner, reflecting their chaperonic activity. All cells constitutively express HSC70. On the other hand, the expression of HSP72 in normal cells is mainly stress inducible, although constitutively high HSP72 expressions are observed in malignant human tumors of various origins. The induced HSP72 expression enables stressed cells to better cope with the elevation of unfolded/denatured proteins and prevent apoptosis under various stress conditions.

Specifications

• Antigen: HSPA8/Hsc70
• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
• Classification: Monoclonal
• Clone: N69
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Accession No.: P19120
• Gene Symbols: HSPA8, HSC70
• Host Species: Mouse
• Immunogen: Co-purified Hsc73 and Hsp72 proteins from bovine skeletal muscle.
• Purification Method: Protein G purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Epigenetics, Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_776770
• Test Specificity: Clone N69 reacts with the constitutively expressed HSPA8/Hsc70 (HSC73), but not the heat schock-inducible HSP72 (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449).
• Target Species: Bovine, Human, Mouse, Rat
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only