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I-A/I-E Rat anti-Mouse, BB515, Clone: 2G9, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 565254

Catalog No. BDB565254

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The 2G9 antibody reacts with the I-Ad and I-Ed MHC class II alloantigens. It also reacts with transfectants expressing I-Eb and I-Ek and with cells from mice of the H-2p and H-2q haplotypes. This antibody is non-reactive with cells from SJL (H-2s) and NOD (H-2g7) mice. Flow cytometric analysis indicates that the 2G9 and M5/114.15.2 (Cat. No. 556999, for purified mAb) monoclonal antibodies have comparable reactivity on cells from mice with I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek alloantigens.

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g. 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.



Aqueous buffered solution containing ≤0.09% sodium azide
IgG2a, κ
Flow Cytometry
H-2I; Ia Ag; I-Ad, I-Ed, I-Eb, and I-Ek MHC class II alloantigens
Affinity purified
BALB/c mouse epidermal Langerhans cells
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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