I-A/I-E Rat anti-Mouse, V500, Clone: M5/114.15.2, BD

Catalog No.	Quantity
BDB562366	50 μg

Catalog No. BDB562366 Supplier BD Biosciences Supplier No. 562366

Description

Rat Monoclonal Antibody

The M5/114.15.2 monoclonal antibody recognizes a polymorphic determinant shared by the I-A[b], I-A[d], I-A[q], I-E[d], and I-E[k] (but not I-A[f], I-A[k], or I-A[s]) MHC class II alloantigens that can be expressed by B cells, dendritic cells, monocytes, macrophages and activated T cells. It also reacts with cells from mice of the H-2[p] and H-2[r] haplotypes, and it is non-reactive with cells from NOD (H-2[g7]) mice. Flow cytometric analysis indicates that the M5/114.15.2 and 2G9 monoclonal antibodies have comparable reactivity on cells from mice with I-A[b], I-A[d], I-A[g7], I-A[q], I-E[d], and I-E[k] alloantigens.

The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser with an Ex max of 415nm and Em Max at 500 nm. BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel. For more information on BD Horizon V500, visit bdbiosciences.com/colors. When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls. Due to spectral differences between cells and beadsin this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents.Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended.Different V500 reagents (e.g. CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

Specifications

• Antigen: I-A/I-E
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: M5/114.15.2
• Concentration: 0.2mg/mL
• Conjugate: Violet Fluor 500 (V500)
• Description: M5/114; H-2I; Ia Ag; I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloAgs
• Formulation: Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.
• Host Species: Rat
• Immunogen: Activated C57BL/6 mouse spleen cells
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2b κ