MilliporeSigma™ anti-ICAM-1 Clone: W-CAM-1,

Catalog No.	Quantity	Conjugate
CP53100UG	100 μg	Unconjugated

Catalog No. CP53100UG Supplier MilliporeSigma™ Supplier No. CP53100UG

Description

Mouse Monoclonal Antibody

Specifically detects ICAM-1 Clone: W-CAM-1 in Human samples, and it is validated for Flow Cytometry, Immunofluorescence, Immunohistochemistry (Frozen), Immunoprecipitation, Inhibition Assays, Immuno Blotting

Cell adhesion molecules are membrane glycoproteins that play a major role in inflammation and neoplastic disease. Four primary groups of adhesion molecules have been described, which includes the integrin receptor family, the immunoglobulin superfamily, selectins, and cadherins. The intercellular adhesion molecules (ICAM-1, ICAM-2, and ICAM-3) belong to the immunoglobulin superfamily that are the counter receptors for the leukocyte integrin, lymphocyte function associated antigen 1 (LFA-1, CD11a/CD18). ICAM-1 (CD54) is a single chain glycoprotein with 7 potential N-linked glycosylation sites. It is expressed in an inducible dependent manner by endothelial and epithelial cells as well as fibroblasts, macrophages, lymphocytes, and leukocytes. ICAM-1 can mediate adhesion by binding to CD11b/CD18 (Mac-1) enhancing antigen specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected erythrocytes distinct from the LFA-1 binding site. Studies indicate that ICAM-1 may also be involved in the development of atherosclerotic plaques; increased expression of ICAM-1 has been observed in all types of atherosclerotic lesions except fibrous placques. It is thought that endothelial cell expression of ICAM-1 mediates the recruitment of leukocytes to the lesion as it is suggested by its role in inflammation.

Specifications

• Antigen: ICAM-1
• Applications: Flow Cytometry, Immunoblot, Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunoprecipitation, Inhibition Assays
• Classification: Monoclonal
• Clone: W-CAM-1
• Conjugate: Unconjugated
• Formulation: In 10mM PBS, 0.2% BSA, pH 7.4.
• Host Species: Mouse
• Immunogen: Raji cells
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the ∽85-115kDa ICAM-1 protein in endothelial cells and tonsil tissue.
• Target Species: Human
• Content And Storage: 2°C to 8°C
• Form: Purified
• Isotype: IgG2b

For Research Use Only