IFN-Gamma Rat anti-Mouse, Brilliant Violet 650, Clone: XMG1.2, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 563854
The XMG1.2 monoclonal antibody specifically binds to mouse interferon- γ(IFN- γ) protein. IFN- γis a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN- γproduction is associated with the Th1 cell differentiation. The purified form of this antibody has been reported to be a neutralizing antibody.
The antibody was conjugated to BD Horizon BV650 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 650|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|IFN-γ; IFN-g; IFN-gamma; Interferon gamma; Type II Interferon|
|Mouse IFN-Gamma Recombinant Protein|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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