IFN-Gamma Rat anti-Mouse, Brilliant Violet 650, Clone: XMG1.2, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 563854
The XMG1.2 monoclonal antibody specifically binds to mouse interferon- γ(IFN- γ) protein. IFN- γis a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN- γproduction is associated with the Th1 cell differentiation. The purified form of this antibody has been reported to be a neutralizing antibody.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 650|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|IFN-γ; IFN-g; IFN-gamma; Interferon gamma; Type II Interferon|
|Mouse IFN-Gamma Recombinant Protein|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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