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Description
Clone 4S.B3 reacts with the human form of interferon-γ (IFN-γ) detectable in the cytoplasm of a major subset of activated peripheral blood T lymphocytes. Clone 4S.B3 also cross-reacts with a cytoplasmic component of peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following five-hour treatment with phorbol myristic acetate (PMA) and Ca++ ionophore (A23187) in the presence of monensin. The staining pattern of 4S.B3 in CD3+ cells is similar to that observed with peripheral blood T lymphocytes from normal human donors. This reagent is useful for intracellular immunofluorescent staining for flow cytometric analysis to identify and enumerate IFN-γ+ cells within a mixed cell population. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Specifications
Specifications
| Antigen | IFN-γ |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | 4S.B3 |
| Conjugate | FITC |
| Formulation | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Partially purified human IFN-γ from supernatants of human PBMC stimulated with Staphylococcus aureus |
| Purification Method | Affinity Purified |
| Quantity | 50 Tests |
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For Research Use Only.
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