IFN-γ Mouse anti-Human, FITC, Clone: 4S.B3, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 552882

Catalog No. BDB552882

Description

Clone 4S.B3 reacts with the human form of interferon-γ (IFN-γ) detectable in the cytoplasm of a major subset of activated peripheral blood T lymphocytes. Clone 4S.B3 also cross-reacts with a cytoplasmic component of peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following five-hour treatment with phorbol myristic acetate (PMA) and Ca++ ionophore (A23187) in the presence of monensin. The staining pattern of 4S.B3 in CD3+ cells is similar to that observed with peripheral blood T lymphocytes from normal human donors. This reagent is useful for intracellular immunofluorescent staining for flow cytometric analysis to identify and enumerate IFN-γ+ cells within a mixed cell population. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: IFN-γ
• Classification: Monoclonal
• Conjugate: FITC
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 4S.B3
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Partially purified human IFN-γ from supernatants of human PBMC stimulated with Staphylococcus aureus
• Quantity: 50 Tests
• Research Discipline: Immunology
• Target Species: Baboon, Cynomolgus, Rhesus Monkey
• Isotype: IgG1 κ

For Research Use Only.