Ig Goat anti-Mouse, Brilliant Violet 421, Polyclonal, BD
Goat Polyclonal Antibody
Manufacturer: BD Biosciences 563846
BD Horizon™ BV421 Goat Anti-Mouse Ig is intended to be a second-step reagent for staining of cells pre-stained with primary mouse Ig antibodies. It is reactive with the heavy and light chains of mouse primary antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes. Minimal background staining of human and rat leucocytes occurs in the absence of a primary mouse antibody. In addition, the BD Horizon BV421 Goat Anti-Mouse Ig stains mouse peripheral B lymphocytes with little non-specific staining of other splenic leucocytes. Therefore, it is useful as a primary reagent in immunofluorescent staining of mouse B cells and antibody-producing cells and as a secondary reagent for staining cells pre-stained with mouse Ig primary antibodies.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Violet 421|
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