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Description
The rat anti-mouse IgE antibody (clone R35-72) reacts specifically with mouse IgE of the Igh-C [a] and Igh-C [b] haplotypes. It has been reported not to react with other Ig isotypes. Detection with the rat anti-mouse IgE antibody (clone R35-72) of surface immunoglobulin on IgE-secreting hybridoma cells has also been reported.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.
Specifications
Specifications
| Antigen | IgE |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | R35-72 |
| Concentration | 0.2mg/mL |
| Conjugate | Brilliant Violet 421 |
| Description | Igh-7; immunoglobulin heavy chain 7 (heavy chain of IgE) |
| Host Species | Rat |
| Immunogen | Mouse IgE (pooled) |
| Purification Method | Affinity Purified |
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