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Description
The C11.5 monoclonal antibody specifically binds to the human IL-12 p40 monomer and p70 heterodimer, but does not bind to the IL-12 p35 monomer. The immunogen used to generate the C11.5 hybridoma was the CHO-expressed recombinant human IL-12 p70 heterodimer. p40 has also been described as a subunit of IL-23 and thus it is possible that the C11.5 antibody crossreacts with IL-23.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
Specifications
Specifications
| Antigen | IL-12 (p40/p70) |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | C11.5 |
| Conjugate | Violet Fluor 450 (V450) |
| Description | IL12B; IL-12B; CLMF; CLMF2; NKSF; NKSF2 |
| Formulation | Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | CHO-expressed recombinant human IL-12 p70 heterodimer |
| Purification Method | Affinity Purified |
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