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IL-2 Rat anti-Human, FITC, Clone: MQ1-17H12, BD

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Catalog No. BDB559361 Supplier BD Biosciences Supplier No. 559361
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Description

Rat Monoclonal Antibody

Clone MQ1-17H12 reacts with the human form of interleukin-2 (IL-2) detectable in the cytoplasm of a major subset of activated peripheral blood T lymphocytes, following treatment with monensin or brefeldin A. Clone MQ1-17H12 also cross-reacts with a cytoplasmic component of peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following six-hour treatment with phorbol myristic acetate (PMA) and Ca++ ionophore (A23187) in the presence of monensin. While the frequency of CD3+ non-human primate cells reacting with MQ1-17H12 following PMA + Ca++ ionophore activation is similar to that observed with normal human donor CD3+ lymphocytes, the fluorescence intensity is characteristically weaker. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Intracellular Staining

TRUSTED_SUSTAINABILITY

Specifications

Antigen IL-2
Applications Flow Cytometry
Classification Monoclonal
Clone MQ1-17H12
Conjugate FITC
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Rat
Immunogen Recombinant Human IL-2
Purification Method Affinity Purified
Quantity 50 Tests
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Target Species Baboon, Cynomolgus, Rhesus Monkey
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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