IL-27 p28 (IL-30) Mouse anti-Mouse, NA/LE, Unlabeled, Clone: MM27.7B1, BD

Catalog No.	Quantity
BDB563284	50 μg

Catalog No. BDB563284 Supplier BD Biosciences Supplier No. 563284

Description

Mouse Monoclonal Antibody

The MM27.7B1 monoclonal antibody specifically binds to the p28 subunit of mouse Interleukin- 27 (IL-27 p28) that is also known as IL-27 alpha (IL-27α) or Interleukin-30 (IL-30). IL-27 p28 is a member of the IL-6/IL-12 family of cytokines. The IL-27 p28 subu nit binds to the EBI3 (Epstein-Barr virus induced gene 3) protein to form heterodimeric Interleukin-27. IL-27 p28 is produced by macrophages and dendritic cells stimulated by inflammatory cytokines and ligands for Toll-like receptors (TLR). IL-27 binds to and transduces signal through surface IL-27 receptors (IL-27R) that are expressed by B cells and naοve T cells and more highly expressed by NK cells and activated T cells. The IL-27R complex is comprised of gp130 (CD130) and WSX-1 (IL-27R alpha) subunits. IL-27 signals through IL-27R that activate the Jak/STAT signaling pathway resulting in the phosphorylation of Stat1 and Stat3. It acts in a proinflammatory manner to upregulate cellular T-bet transcription fac tor and surface IL-12R beta2 expression contributing to higher levels of IFN-γand granzyme B expression. IL-27 exerts anti-inflammatory responses by suppressing the differentiation and proliferation of Th2 and Th17 cells. IL-27 p28 is reportedly produced and secreted independently of EBI3. It may inhibit the actions of IL-17 and other proinflammatory cytokines like IL-6. The MM27/7B1 antibody has been reported to neutralize IL-27 activity in vitro. Routine quality testing of mAb MM27.7B1 is performed via flow cytometry of intracellularly stained IL-27 p28-transfected cells.

Specifications

• Antigen: IL-27 p28 (IL-30)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: MM27.7B1
• Concentration: 1.0mg/mL
• Conjugate: Unconjugated
• Description: Il27; IL-27 alpha; Il27a; IL27-A; IL-27-A; IL-27α; IL-27 p28; Il30; IL-30
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Host Species: Mouse
• Immunogen: Mouse IL-27
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C
• Isotype: IgG2a κ