IL-27 p28 (IL-30) Mouse anti-Mouse, NA/LE, Unlabeled, Clone: MM27.7B1, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 563284

Catalog No. BDB563284

Description

The MM27.7B1 monoclonal antibody specifically binds to the p28 subunit of mouse Interleukin- 27 (IL-27 p28) that is also known as IL-27 alpha (IL-27α) or Interleukin-30 (IL-30). IL-27 p28 is a member of the IL-6/IL-12 family of cytokines. The IL-27 p28 subu nit binds to the EBI3 (Epstein-Barr virus induced gene 3) protein to form heterodimeric Interleukin-27. IL-27 p28 is produced by macrophages and dendritic cells stimulated by inflammatory cytokines and ligands for Toll-like receptors (TLR). IL-27 binds to and transduces signal through surface IL-27 receptors (IL-27R) that are expressed by B cells and naοve T cells and more highly expressed by NK cells and activated T cells. The IL-27R complex is comprised of gp130 (CD130) and WSX-1 (IL-27R alpha) subunits. IL-27 signals through IL-27R that activate the Jak/STAT signaling pathway resulting in the phosphorylation of Stat1 and Stat3. It acts in a proinflammatory manner to upregulate cellular T-bet transcription fac tor and surface IL-12R beta2 expression contributing to higher levels of IFN-γand granzyme B expression. IL-27 exerts anti-inflammatory responses by suppressing the differentiation and proliferation of Th2 and Th17 cells. IL-27 p28 is reportedly produced and secreted independently of EBI3. It may inhibit the actions of IL-17 and other proinflammatory cytokines like IL-6. The MM27/7B1 antibody has been reported to neutralize IL-27 activity in vitro. Routine quality testing of mAb MM27.7B1 is performed via flow cytometry of intracellularly stained IL-27 p28-transfected cells.

Specifications

• Antigen: IL-27 p28 (IL-30)
• Clone: MM27.7B1
• Conjugate: Unconjugated
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Host Species: Murine
• Isotype: IgG_2a, κ
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

• Applications: Flow Cytometry
• Concentration: 1.0mg/mL
• Description: Il27; IL-27 alpha; Il27a; IL27-A; IL-27-A; IL-27α; IL-27 p28; Il30; IL-30
• Format: Affinity Purified
• Immunogen: Mouse IL-27
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C
• Monoclonal or Polyclonal: Monoclonal