IL-4 Rat anti-Mouse, Brilliant Violet 711, Clone: 11B11, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 564005
The 11B11 monoclonal antibody specifically binds to mouse Interleukin-4 (IL-4). The immunogen used to generate the 11B11 hybridoma was partially purified mouse IL-4 prepared from the supernatant of Phorbol 12-Myristate 13-Acetate (PMA)-stimulated EL-4 cells. The 11B11 antibody is reportedly a neutralizing antibody.
The antibody was conjugated to BD Horizon BV711 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy5.5 / Alexa Fluor 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 711|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|IL4: Interleukin-4; BSF-1; B-cell growth factor 1; BCGF-1|
|Partially Purified Mouse IL-4|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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