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IL-4 Rat anti-Human, Brilliant Violet 711, Clone: MP4-25D2, BD
Rat Monoclonal Antibody
Supplier: BD Biosciences 564112
Description
The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications
IL-4 | |
Monoclonal | |
Brilliant Violet 711 | |
Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
Purified Recombinant Human IL-4 | |
100 Tests | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Flow Cytometry | |
MP4-25D2 | |
Interleukin-4; IL4; BCGF-1; BSF-1; Lymphocyte stimulatory factor 1 | |
Rat | |
Affinity Purified | |
RUO | |
Human | |
IgG1 |
Safety and Handling
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