INF-g Mouse anti-Human, PE-Cy7, Clone: B27, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 560924

Catalog No. BDB560924

Description

The B27 monoclonal antibody specifically binds to human interferon-γ (IFN-γ). IFN-γ is a potent multifunctional cytokine that is produced by several activated cell types including NK, NKT, CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells. IFN-γexerts its biological effects through specific binding to the high-affinity IFN-γ Receptor Complex comprised of IFN-γβ subunits. In addition to its antiviral effects, IFN-γupregulates a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γcan exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ’s capacity to boost MHC class I and II expression by antigen-presenting cells as well as to direct effects on B cells and T cells themselves. Human IFN-γis a 14-18 kDa glycoprotein containing 143 amino acid residues. B27 is a neutralizing antibody. The use of B27 antibody for epitope mapping of human IFN-γ has been described. The B27 antibody has been reported not to bind to denatured IFN-γ.

Specifications

• Antigen: INF-g
• Classification: Monoclonal
• Conjugate: PE-Cyanine7
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: B27
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Human IFN-γ Recombinant Protein
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.