MilliporeSigma™ anti-Integrin beta1 Alexa Fluor 488, Clone: HUTS-4,

Catalog No.	Quantity	Conjugate
MAB2079AF48	100 μL	Alexa Fluor 488

Catalog No. MAB2079AF48 Supplier MilliporeSigma™ Supplier No. MAB2079AF488

Description

Mouse Monoclonal Antibody

Specifically detects Integrin β1 Clone: HUTS-4 in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

Specifications

• Antigen: Integrin β1
• Applications: Immunocytochemistry
• Classification: Monoclonal
• Clone: HUTS-4
• Conjugate: Alexa Fluor 488
• Formulation: Purified mouse antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
• Gene Accession No.: P05556
• Gene Symbols: ITGB1, FNRB, MDF2, MSK12
• Host Species: Mouse
• Immunogen: Generated against human β1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues.
• Purification Method: Protein G purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NM_002292.3
• Test Specificity: Clone HUTS-4 is specific for active conformation of human beta1 integrins. The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit nonconvalently associated with the common beta1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg²⁺ or Mn²⁺, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. Recognizes epitopes in the 355-425 region of the common beta1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli.
• Target Species: Human, Mouse, Rat
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Form: Purified
• Isotype: IgG2a

For Research Use Only