Itch Mouse, Unlabeled, Clone: 32, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 611198

Catalog No. BDB611198

Description

Maintenance of cellular function requires timely and selective degradation of key regulatory proteins. For example, progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins via the ubiquitin pathway. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an ε-NH2-Lys in a target protein. Ubiquitin-conjugated proteins are recognized and degraded by the 26S proteasome. Ubiquitination requires ubiquitin-activating enzyme E1, ubiquitin-conjugating enzymes E2, and ubiquitin ligases E3. The direction of ubiquitin transfer is from E1 to E2 and from E2 to E3. Itch, a novel E3 ubiquitin ligase, is absent in the Non-agouti-lethal 18H mice. These mice develop immunological, inflammatory, epithelial, and hematopoietic diseases. Itch contains four WW protein interaction domains, which bind to proline-rich sequences in a fashion similar to SH3 domains. In addition, Itch contains a C-terminal Hect domain, which is conserved in the E3 family of ubiquitin ligases. Thus, Itch is important in the ubiquitin-dependent protein degradation occurring in normal hematopoiesis and inflammation.

Host Species: Mouse
Clone: 32
Isotype: IgG1
Species Reactivity: Rat
Immunogen: Mouse Itch aa. 114-220
Formula Weight [Chemical]: 113kDa

Immunofluorescence, Western Blotting

Specifications

• Antigen: Itch
• Clone: 32
• Conjugate: Unconjugated
• Format: Affinity Purified
• Immunogen: Mouse Itch aa. 114-220
• Quantity: 50μg
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Human, Murine, Rabbit, Rat

• Applications: Western Blot
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Host Species: Murine
• Isotype: IgG₁
• Regulatory Status: RUO
• Storage Requirements: Store undiluted at -20°C.
• Monoclonal or Polyclonal: Monoclonal

For Research Use Only.