MilliporeSigma™ anti-KAP-1/TRIM28, Polyclonal

Catalog No.	Quantity	Conjugate
ABE1859MI	100 μL	Unconjugated

Catalog No. ABE1859MI Supplier MilliporeSigma™ Supplier No. ABE1859

Description

Rabbit Polyclonal Antibody

Specifically detects KAP-1/TRIM28 Clone: in Human, Mouse samples, and it is validated for Immunocytochemistry, Immunoprecipitation, Western Blotting

Transcription intermediary factor 1-beta (UniProt Q13263; also known as E3 SUMO-protein ligase TRIM28, KAP-1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, Protein phosphatase 1 regulatory subunit 157, RING finger protein 96, TIF1-beta, Transcriptional intermediary factor 1-beta, Tripartite motif-containing 28, Tripartite motif-containing protein 28) is encoded by the TRIM28 (also known as KAP1, PPP1R157, RNF96, TF1B, TIF1B) gene (Gene ID 10155) in human. Kruppel-associated box-/KRAB-associated protein 1 (KAP-1) is a transcriptional co-repressor that selectively targets the KRAB domain of zinc finger protein-based transcription factors with its N-terminal tripartite RBCC (RING, b-box, coiled-coil) domain. KAP-1 uses its C-terminal PxVxL motif and the PHD-Bromo domain to recruit chromatin modifiers, such as heterochromatin protein 1 (HP-1), Histone-lysine N-methyltransferase SETDB1, nucleosome remodeling and deacetylation (NuRD) complex, and histone deacetylases (HDACs), for transcriptional repression. KAP-1 also plays an essential role in the DNA damage response (DDR). Upon DNA damage, KAP-1 is rapidly phosphorylated by Ataxia telangiectasia mutated (ATM) on Ser824, causing chromatin relaxation to facilitate DNA repair. In addition, SIRT1-mediated KAP-1 deacetylation is reported to stabilize KAP1 and 53BP1 interaction, resulting in an enhancement of NHEJ-mediated repair efficiency in a KAP-1 pSer824-independent manner.

Specifications

• Antigen: KAP-1/TRIM28
• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Rabbit polyclonal antibody in serum with 0.05% sodium azide.
• Gene Accession No.: Q13263
• Gene Symbols: TRIM28, KAP1, RNF96, TIF1B
• Host Species: Rabbit
• Immunogen: GST-tagged recombinant protein corresponding to the Bromo domain of human KAP-1/TRIM28.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Epigenetics, Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_005753
• Test Specificity: Expected to react with both spliced isoforms.
• Target Species: Human, Mouse
• Content And Storage: −20°C for one year
• Form: Serum

For Research Use Only