MilliporeSigma™ anti-KIF1B Clone: 10F3.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC309

Catalog No. MABC309MI

Description

Specifically detects KIF1B Clone: 10F3.1 in Human, Mouse, Rat samples, and it is validated for Immunohistochemistry (Paraffin), Western Blotting

Kinesin-like protein KIF1B (UniProt O60333; also known as Kinesin superfamily protein KIF1B, Klp) is encoded by the KIF1B (also known as KLP, CMT2, CMT2A, CMT2A1, HMSNII, KIAA0591, KIAA1448, NBLST1) gene (Gene ID 23095) in human. KIF1B is a member of the kinesin 3 family of proteins. The KIF1B gene encodes four alternatively spliced isoforms, among which isoform 2 and isoform 3 are also known as the alpha and beta isoforms. The alpha and beta isoforms share a common N-terminal motor domain but contain different C-terminal cargo domains. The alpha and beta isoforms are motor proteins implicated in anterograde transport of mitochondria and synaptic vesicle precursors, respectively. In addition, the beta isoform is required for developmental apoptosis induction by nerve growth factor (NGF) deprivation among neural crest progenitors during sympathetic neuron development. The beta isoform is reported to interact with RNA helicase A (DHX9), resulting in DHX9 nuclear accumulation and upregulated transcription of the proapoptotic XIAP-associated factor 1 (XAF1). KIF1B gene is located in a chromosomal region frequently deleted in neural crest-derived tumors, including neuroblastomas with loss of KIF1Bβ expression and impaired DHX9 nuclear localization.

Specifications

• Antigen: KIF1B
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Accession No.: O60333
• Host Species: Mouse
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Expected to react with spliced isoforms 1 and 2 (isoform Beta), but not isoform 3 (isoform Alpha). Reactivity toward isoform 4 is highly likely, but not yet confirmed.
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Isotype: IgG2a κ

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: 10F3.1
• Formulation: Mouse monoclonal IgG2aκ tissue culture supernatant without preservatives.
• Gene Symbols: KIF1B, KIAA0591, KIAA1448
• Immunogen: GST-tagged recombinant protein corresponding to the C-terminal region of human KIF1B.
• Quantity: 100 μL
• Research Discipline: Apoptosis, Cancer
• Gene ID (Entrez): NP_055889
• Target Species: Human, Mouse, Rat
• Form: Supernatant

For Research Use Only