KLRG1 Hamster anti-Mouse, Alexa Fluor 488, Clone: 2F1, BD

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 561619

Catalog No. BDB561619

Description

The 2F1 monoclonal antibody specifically binds to KLRG1 (Killer cell Lectin-like R), which is the mouse homologue of the rat mast cell function-associated antigen (MAFA),on all mouse strains tested (eg, AKR/J, BALB/c, C3H/HeN, C3H.SW, C57BL/6, DBA/1, SJL, 129/J). Unlike rat MAFA, which is expressed on mast cells, mouse KLRG1 is expressed on a large subset of NK cells, lymphokine-activated killer (LAK) cells, adherent LAK (A-LAK) cells, subsets of activated CD8+ T lymphocytes, and small fractions of CD4+ and CD8+ T cells, but not mast cells. The expression of KLRG1 is correlated with reduced proliferative capacity of activated T lymphocytesor reduced effector functions of activated NK cells. This molecule is believed to play a common role in the regulation of leukocytes of both the innate and adaptive immune system. It has been observed that the 2F1 mAb stains the rat basophilic leukemia cell line, RBL-2H3, which is known toexpress MAFA. The KLRG1 protein is an inhibitory lectin-like type IItransmembrane receptor containing a cytoplasmic motif similar to ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif);its ligand has not beenidentified KLRG1 is expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kDa. The level of KLRG1 expression is reduced in MHC class I-deficient mice, although direct binding of KLRG1 to MHC class I antigens could not be detected. Cross-linking of KLRG1 by 2F1 mAb reduces TCR-mediated Ca++mobilization and cytotoxic responses (but not IFN-γ production) by CD8+ T cellsand inhibits IFN-γ

Specifications

• Antigen: KLRG1
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Klrg1; Killer cell lectin-like receptor subfamily G member 1; MAFA
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2 κ

• Applications: Flow Cytometry
• Clone: 2F1
• Conjugate: Alexa Fluor 488
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: A-LAK from C57BL/6 mice
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.