KSR-1 Mouse, Unlabeled, Clone: 15, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 611576

Catalog No. BDB611576

Description

Proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. In mammalian cells there are four true Ras proteins (encoded by Ha-ras, N-ras, Ki-rasA, and Ki-rasB). These proteins relay signals from tyrosine kinases at the plasma membrane to the nucleus via a network of Ser/Thr kinases that includes the MAP kinase (Raf-MEK-ERK) pathway. Kinase suppressor of Ras (KSR-1) was discovered in a genetic screen to identify mutations that suppress constitutively active Ras mutants. KSR-1 contains five domains that are conserved areas (CA) in Drosophila, mouse, and human. CA1 is a unique domain, CA2 is a proline-rich domain, CA3 is a cysteine-rich domain, CA4 is a Ser/Thr rich region, and CA5 contains a C-terminal kinase domain similar to that of Raf. In COS7 cells, ceramide induces the autophosphorylation of KSR-1 and KSR-1-induced phosphorylation and activation of Raf. In addition, overexpression of KSR-1 in 293T cells leads to recruitment of MEK to a 700kDa protein complex and translocates MEK from the soluble to the membrane-associated fraction. Thus, KSR-1 may have important kinase and scaffolding activities during Ras-related signaling.

Host Species: Mouse
Clone: 15
Isotype: IgG1
Species Reactivity: Mouse
Immunogen: Mouse KSR-1 aa. 90-203
Formula Weight [Chemical]: 115kDa

Immunofluorescence, Western Blotting

Specifications

• Antigen: KSR-1
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Mouse KSR-1 aa. 90-203
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Mouse, Rat
• Isotype: IgG1

• Applications: Western Blot
• Clone: 15
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.