MilliporeSigma™ anti-Laminin-5 Alexa Fluor 647, Clone: D4B5,

Catalog No.	Quantity	Conjugate
MAB19562AF6	100 μL	Alexa Fluor 647

Catalog No. MAB19562AF6 Supplier MilliporeSigma™ Supplier No. MAB19562AF647

Description

Mouse Monoclonal Antibody

Specifically detects Laminin-5 Clone: D4B5 in Human, Mouse samples, and it is validated for Immunocytochemistry

Laminin subunit gamma-2 (UniProt Q13753; also known as CSF 140kDa subunit, Cell-scattering factor 140kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Ladsin 140kDa subunit, Laminin B2t chain, Lamin-5 subunit gamma, Large adhesive scatter factor 140kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as B2T, LAMB2T, LAMNB2) gene (Gene ID 3918) in human. Laminins are basement membrane, extracellular matrix glycoproteins composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes. Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease. LN5 may stabilize the epidermal/dermal junction of skin through interaction with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. Comparing to fibronectin, collagen, and vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent. Laminin-5 attracted attention when it was detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, T., et al. (2002). Am. J. Pathol. 160(3):112911-41; Lohi, J. (2001). Int. J. Cancer. 94(6):763-767).

Specifications

• Antigen: Laminin-5
• Applications: Immunocytochemistry
• Classification: Monoclonal
• Clone: D4B5
• Conjugate: Alexa Fluor 647
• Formulation: Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.
• Gene Accession No.: Q13753-1
• Gene Symbols: LAMC2, LAMB2T, LAMNB2
• Host Species: Mouse
• Immunogen: Amino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein.
• Purification Method: Protein A purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_005553
• Test Specificity: This antibody recognizes the 150 (precursor form) and 105kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.
• Target Species: Human, Mouse
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Form: Purified
• Isotype: IgG1

For Research Use Only