MilliporeSigma™ anti-LBPA Clone: 6C4,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABT837

Catalog No. MABT837MI

Description

Specifically detects LBPA Clone: 6C4 in All samples, and it is validated for Dot Blot, Electron Microscopy, ELISA, Immunocytochemistry

Lyso bis-phosphatidic acid (LBPA, Lysobisphosphatidic acid, L(bis)PA) is a lipid that is exclusively located in late endosomes and is used as a late endosome marker. One of the characteristic features of late endosomes is a complex system of internal membranes within the lumen that contains large amounts of the unique, poorly degradable LBPA, and thus forms a specialized membrane domain within endosomes. Late endosomes function not only as a major protein-sorting compartment, but also an obligatory station for LDL and other endocytosed ligands destined for degradation. Research shows that the LBPA-rich membranes within late endosomes regulate cholesterol transport, presumably by acting as a collection and distribution device. The genetic disease Niemann-Pick type C (NPC) is characterized by both lysosomal and endosomal storage disorders. In skin fibroblasts from NPC patients, cholesterol accumulation is seen within vesicles containing late endosome markers LBPA and Rab7.

Specifications

• Antigen: LBPA
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: All species
• Form: Purified

• Applications: Dot Blot, Immunoassay, ELISA, Immunocytochemistry
• Clone: 6C4
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Immunogen: Endosomal membranes from baby hamster kidney fibroblasts (BHK) corresponding to Hamster LBPA.
• Quantity: 100 μg
• Research Discipline: Cell Structure
• Test Specificity: Clone 6C4 specifically detects LBPA, but not PC, PE, SM, PS, CL, PI, Sul, Chol, Cer, or CE purified from baby hamster kidney (BHK) cell lipid extract (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197).
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgG1 κ

For Research Use Only