LIMD1 Rabbit anti-Human, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA532475

Catalog No. PIPA532475

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is lung tissue.

The Zyxin family of proteins contains five members: Ajuba, Limd1, LPP, TRIP6 and Zyxin. Limd1 (LIM domain-containing protein 1) is a ubiquitously expressed tumor suppressor containing three LIM zinc-binding domains. LIM domains consist of a cysteine-rich consensus sequence containing two distinct zinc-binding subdomains, which mediate protein-protein interactions. Limd1 interacts with the proteins SQSTM1, Rb, p62 and TRAF6. Limd1 was first identified when the deletion of its gene was noted in some cervical cancers. Limd1 blocks in vitro and in vivo tumor growth and is downregulated in lung cancer.

Specifications

• Antigen: LIMD1
• Classification: Polyclonal
• Gene: LIMD1
• Gene Alias: LIM domain-containing protein 1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8994
• Content And Storage: 4° C, do not freeze
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Gene Accession No.: Q9UGP4
• Gene Symbols: LIMD1
• Immunogen: Synthetic peptide derived from N-terminus of human LIMD1 protein.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG