Ly-6A/E Rat anti-Mouse, Brilliant Violet 786, Clone: D7, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 563991
The D7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca-1 (Ly6A/E), a phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cells (HSC) in the bone marrow of mice with both Ly-6 haplotypes. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of lymphocytes from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb (anti-Ly-6A/E, Cat. No. 553333) can block binding of FITC-conjugated D7 antibody (Cat. No. 557405) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated E13-161.7 antibody (Cat. No. 553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Cat. No. 559971) to identify HSC.
The antibody was conjugated to BD Horizon BV786 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy7-like dyes (eg, 780/60-nm filter).
|Brilliant Violet 786|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Ly-6A/E; Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Sca-1; TAP|
|IL-2-dependent mouse T-cell line CTL-L|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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