Ly-6C Rat anti-Mouse, V450, Clone: AL-21, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 560594

Catalog No. BDB560594

Description

The AL-21 antibody reacts with a non-polymorphic determinant of Ly-6C, a 14- 17kDa GPI-linked cell-surface antigen found on some monocyte/macrophage populations, granulocytes, endothelial cells, plasma cells, and thymocyte, NK-cell, and T-subsets. Mice with the Ly-6.2 alloantigen (eg, AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129) have subsets of CD8+ and CD4+ Ly-6C+ T cells, while Ly-6.1 strains (eg, A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+ Ly-6C+ T cells. Upregulation of Ly-6C expression on CD8+ T cells by interferons α and β and poly (I:C) has been described, and Ly-6C is a memory marker on CD8+ T cells. The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Flow Cytometry

Specifications

• Antigen: Ly-6C
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.
• Immunogen: Not reported
• Quantity: 50 μg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgM κ

• Applications: Flow Cytometry
• Clone: AL-21
• Conjugate: Violet Fluor 450 (V450)
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov