MilliporeSigma™ anti-Ly-6G APC, Clone: RB6-8C5,

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABF1478MI	MilliporeSigma™ MABF1478	100 μg

Description

Specifically detects Ly-6G Clone: RB6-8C5 in Mouse samples, and it is validated for Flow Cytometry

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

Specifications

• Antigen: Ly-6G
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Rat
• Research Discipline: Inflammation, Immunology
• Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
• Gene Symbols: Ly6g
• Primary or Secondary: Primary
• Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze

• Clone: RB6-8C5
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Gene Accession No.: P35461
• Immunogen: Proprietary immunogen
• Purification Method: Purified by size-exclusion chromatography

For Research Use Only