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Ly-6G Rat anti-Mouse, Brilliant Violet 711, Clone: 1A8, BD

Catalog No. BDB563979 Shop All BD Biosciences Products
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Catalog No. BDB563979 Supplier BD Biosciences Supplier No. 563979
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Rat Monoclonal Antibody

The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa glycosyolphosphatidylinositol-anchored protein. In the bone marrow, Ly-6G is expressed on the majority of the largest cells, which are predominantly granulocytes, and not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The RB6-8C5 monoclonal antibody recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

TRUSTED_SUSTAINABILITY

Specifications

Antigen Ly-6G
Applications Flow Cytometry
Classification Monoclonal
Clone 1A8
Concentration 0.2mg/mL
Conjugate Brilliant Violet 711
Description Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Rat
Immunogen Ly-6G-transfected EL4J cell line
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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