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Ly-6G Rat anti-Mouse, Brilliant Violet 711, Clone: 1A8, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 563979

Catalog No. BDB563979


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Description

Description

The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa glycosyolphosphatidylinositol-anchored protein. In the bone marrow, Ly-6G is expressed on the majority of the largest cells, which are predominantly granulocytes, and not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The RB6-8C5 monoclonal antibody recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

Specifications

Ly-6G
1A8
Brilliant Violet 711
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Rat
IgG2a, κ
RUO
Primary
Murine
Flow Cytometry
0.2mg/mL
Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
Affinity Purified
Ly-6G-transfected EL4J cell line
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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