LY-6G Rat anti-Mouse, Brilliant Violet 605, Clone: 1A8, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 563005

Catalog No. BDB563005

Description

The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa glycosyolphosphatidylinositol-anchored protein. In the bone marrow, Ly-6G is expressed on the majority of the largest cells, which are predominantly granulocytes, and not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The RB6-8C5 monoclonal antibody recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.

This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

Specifications

• Antigen: LY-6G
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: 1A8
• Conjugate: Brilliant Violet 605
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Ly-6G-transfected EL4J cell line
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.