LY-6G and LY-6C Rat anti-Mouse, V450, Clone: RB6-8C5, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 560454

Catalog No. BDB560454

Description

The RB6-8C5 antibody reacts with a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 mAb is a component of the “lineage cocktail” used in studies of hematopoietic lineages. The mAb 1A8 (Mfr. No. 551461) specifically recognizes Ly-6G, but not Ly-6C. The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Host Species: Rat
Clone: RB6-8C5
Isotype: IgG2b, κ
Species Reactivity: Mouse
Immunogen: Not Reported

Flow Cytometry

Specifications

• Antigen: LY-6G and LY-6C
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Ly6c, Lymphocyte antigen 6C2; Lymphocyte antigen 6G, Ly6g, Gr-1
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: RB6-8C5
• Conjugate: Violet Fluor 450 (V450)
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: Not Reported
• Quantity: 25 μg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2b κ

For Research Use Only.