Maspin Mouse anti-Human, Unlabeled, Clone: G167-70, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 554292

Catalog No. BDB554292

Description

Maspin is a 42kDa protein that may play a role in human breast cancer by functioning as a tumor suppressor. Maspin is expressed in normal mammary epithelial cells, but there is a loss of expression in invasive breast cancer including lymph node and distant metastases. Further, maspin is not expressed in most mammary carcinoma cell lines. Mammary carcinoma cells transfected with recombinant maspin have a reduced ability to migrate through reconstituted basement membrane and lose their ability to form tumors in nude mice, providing support for maspin as a tumor suppressor protein. Maspin has homology to members of the serpin family of protease inhibitors, some of which have been implicated in the regulation of invasion and metastasis. Yet protease inhibitory activity has not been detected using recombinant maspin. It has been suggested that maspin, unlike most serpins, may be a ligand-binding serpin rather than a protease inhibitor. However, the question of whether or not maspin has protease inhibitor activity remains to be determined. G167-70 is specific for human maspin. It detects maspin as a 42kDa protein in western blots of normal human mammary epithelial cells. Many mammary tumor cell lines including MDA-MB-231 (ATCC
HTB 26) and MDA-MB-435S (ATCC HTB 129) lack maspin expression and are suitable as negative controls. Purified recombinant human maspin from a yeast expression system was used as immunogen.

Host Species: Mouse
Clone: G167-70
Isotype: IgG2b
Species Reactivity: Human
Immunogen: Recombinant Human Maspin
Formula Weight [Chemical]: 42kDa

Western Blotting

Specifications

• Antigen: Maspin
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Recombinant Human Maspin
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG2b

• Applications: Western Blot
• Clone: G167-70
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.