KCNMA1 Rabbit anti-Human, Rat, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1923
PA1-923 detects large conductance, calcium-activated potassium channel alpha (Maxi K+ alpha) from human tissues as well as recombit human protein and rat samples. PA1-923 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~110 kDa protein representing Maxi K+ alpha from human myometrial smooth muscle cell extracts. Immunocytochemical staining of Maxi K+ alpha in human coronary muscle cells with PA1-923 results in predomit staining of the plasma membrane. PA1-923 immunizing peptide corresponds to amino acid residues 945-961 from human Maxi K+ alpha. This sequence is completely conserved across many species. PA1-923 immunizing peptide (Cat. No. PEP-113) is available for use in neutralization and control experiments.Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.
|Synthetic peptide corresponding to residues C T(945) E L V N D T N V Q F L D Q D D D(961).|
|Antigen affinity chromatography|
|Immunocytochemistry, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|KCNMA1b, KCNMA1c, Kcnma, Slo|
|-20° C, Avoid Freeze/Thaw Cycles|
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