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MEK1 Mouse, Alexa Fluor 647, Clone: 25/MEK1, BD

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BDB560101 50 Tests
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Catalog No. BDB560101 Supplier BD Biosciences Supplier No. 560101
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Description

Mouse Monoclonal Antibody

MEK1 ( M apK/ E RK K inase ) is a 45-kDa member of the MEK family of dual specificity kinases. MEK is activated by a variety of cellular serine/threonine kinases including c-Raf, A-Raf, c-mos, and MEK Kinase-1. Activated MEK phosphorylates MAP kinase (ERK) at threonine and tyrosine residues. This results in activation of ERK and its signaling pathway. MEK is highly specific for ERK and various MEKs preferentially phosphorylate individual ERK isoforms. MEK1 only activates ERK1 and ERK2. This specificity may result from variations in ERK regions that are known as the phosphorylation lip and kinase backbone. MEK's localization is cytoplasmic, but mitogenic stimulation induces a mass translocation to the nucleus. Mechanisms behind this nuclear translocation remain unknown. However, MEK contains an N-terminal nuclear export signal (NES) that mediates its rapid exodus from the nucleus and restores its unstimulated cellular distribution.

The 25/MEK1 monoclonal antibody recognizes MEK1, regardless of phosphorylation status.

The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.

TRUSTED_SUSTAINABILITY

Specifications

Antigen MEK1
Applications Flow Cytometry
Classification Monoclonal
Clone 25/MEK1
Conjugate Alexa Fluor 647
Description MAPK/ERK kinase 1, EC 2.7.12.2, kinase MEK1, MAPKK1, PRKMK1
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human MEK1 Recombinant Protein
Purification Method Affinity Purified
Quantity 50 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Canine, Chicken, Human, Mouse, Rat
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a
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