MLH1 Mouse anti-Human, Unlabeled, Clone: G168-728, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 554073

Catalog No. BDB554073

Description

The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. Loss of function of DNA repair enzymes can lead to an accumulation of replication errors, resulting in a mutated phenotype. DNA repair enzymes are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex, it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. The G168-728 antibody recognizes human and mouse MLH1 (80-85kDa). Full-length human recombinant MLH was expressed as a maltose binding-MLH fusion protein, affinity purified, and used as immunogen.

Host Species: Mouse
Clone: G168-728
Isotype: IgG2a κ
Species Reactivity: Human
Immunogen: Recombinant Human MLH
Formula Weight [Chemical]: 80-85kDa

Immunoprecipitation, Western Blotting

Specifications

• Antigen: MLH1
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Recombinant Human MLH
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human, Mouse
• Isotype: IgG2a κ

• Applications: Western Blot
• Clone: G168-728
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.